In metabolomics, real endeavours are put resources into the advancement of suitable scientific methodologies. Metabolites includes a diverse group of low-molecular-weight structures such as lipids, nucleic acids,amino acids, peptides, vitamins, organic acids, thiols and carbohydrates, which makes global analysis a big challenge. The recent development of analytical platforms, such as GC, HPLC, UPLC, CE coupled to MS and NMR spectroscopy, enables separation, detection, characterization and quantification of these metabolites and other related metabolic pathways. Because of the complexity of the metabolome and diverse properties of the metabolites, single analytical platform cannot be used for detecting all metabolites in a sample. The combined use of modern instrumental analytical approaches has led to the ideal outcomes, and is important for increasing the coverage of metabolites which cannot be achieved by single-analysis techniques. Integrated platforms are frequently being used for providing sensitive and reliable detection of thousands of metabolites in a bio fluid sample. Continued development of these analytical platforms will accelerate widespread use and integration of metabolomics into systems biology.
- Analytical variability and issues
- Targeted metabolic profiles
- Chromatographic methods in metabolomics