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Fatehmeh Khassafi
Royan Institute for Stem Cell Biology and Technology, Iran

Fatehmeh Khassafi has received her Bachelor degree in Biology at University of Tehran, which is the most reputable university in Iran. She continued her education in Cell and Developmental Biology at University of Science and Culture in collaboration with Royan Institute – established in 1991 – is one of the pioneers in Stem Cell Research, Cell Therapy and Reproductive Biomedicine in Iran.


Metabolic network reconstruction which is one of the valuable methods used to predict phenotype and analysis of cell physiology is the mathematical representation that encompasses all metabolic pathways in a cell or an organism. Genome-scale metabolic networks are primarily reconstructed for specific organisms based on their annotated genomes and then, context specific models are needed to facilitate constraint-based analysis in different eukaryotic cell types. Since pluripotent stem cells can be potentially used in regenerative medicine and disease modeling, investigating their functions has recently been at the centre of attention. Pluripotent stem cells are proliferative, so they have different bioenergetics and biosynthetic demands compared to their differentiated counterparts, although the underlying mechanism of this metabolism is still unclear. Furthermore, different pluripotent states display controversies in some metabolic traits also have been shown during induced pluripotent cell generation. Comprehensive assessment of unique metabolism of pluripotent cell is only possible via in-silico modeling of metabolism which also overcomes the limitations of time-consuming and expensive experiments. Here, we reconstructed different types of mouse pluripotent cell specific metabolic models, using transcriptomic data. Further comparison and analysis will be performed on these models leads to description of metabolic features of mouse pluripotent cell states that cast light on underlying mechanisms of cell fate determination. Confirming this, pave the way for functional assays of metabolism of pluripotent cells and elucidate role of metabolism in regulating pluripotent cell conversion and differentiation into various cell types.

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